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Star Republic: Guide for Biologists

Northern

1. Separate polyA or total RNA on a Formaldehyde-Agarose Gel.

2. Transfer RNA to a hybridization membrane using capillary transfer or vacuum blot. No pre-treatment of the gel is required.

3. Labeling of probe and hybridization procedures are the same for Southern hybridization.

Southern --- radioactive
Southern --- Chemiluminescence detection