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Star Republic: Guide for Biologists

RNA purification --- LiCl precipitation

Lithium chloride does not precipitate tRNA, and is less efficient for RNA smaller than 300 nucleotides. It is also less efficient in removing DNA compared to acid phenol:chloroform extraction.

1. Add equal volume of 7.5 M LiCl to a solution containing RNA.

2. Mix and precipitate at -20 C for at least 30 minutes.

3. Centrifuge at maximum speed for 10 minutes in a microcentrifuge.

4. Carefully remove supernatant. Wash the pellet with 70% ethanol.

5. Carefully remove supernatant. Air dry and resuspend in a suitable buffer. Store at -20 C or -80 C.