Diethylpyrocarbonate (DEPC) is commonly used to treat water
and other solutions for destroying RNase contaminations. Note reagents
containing amine groups (e.g., Tris and HEPES) may not be treated with
DEPC, since amine groups tend to react with DEPC.
1. Add DEPC to water or solution to the final concentration of 0.1% DEPC.
2. Incubate at room temperature overnight.
3. Autoclave the solution to break down DEPC.