1. HRP substrates include
diaminobenzidine (DAB), tetramethylbenzidine (TMB), p-PHENYLENEDIAMINE DIHYDROCHLORIDE/pyrocatechol,
3-AMINO-9-ETHYLCARBAZOLE (AEC), 4-CHLORO-1-NAPHTHOL (CN).
2. HRP is frequently coupled to antibodies for histochemistry and ELISA.
3. HRP/DAB is used in the ABC staining.
4. HRP/TMB is frequently used in ELISA.
5. HRP is frequently used to label neurons. Damaged neurons readily
uptake HRP, allowing neuronal structures being labeled by a HRP substrate.