Science Gateway > Protocols > Cell Biology Protocols - Table of Contents - a PubMed search engine
Star Republic: Guide for Biologists

Alkaline phosphatase
1. Alkaline phosphatase (AP) is frequently coupled to secondary antibodies for imunohistochemical staining, ELISA, and calorimetric Western/Southern/Northern.

2. AP is an important histological marker.

3. A heat stable AP has been used as a reporter gene for transfection or transgenic. The endogenous AP will be inactivated by incubating samples at 65 C for 30 minutes.

4. AP can be assayed using supernatants from transfected cells when secreted.

5. AP is also used for dephosphorylation of restriction digestion ends (see CIP and SAP).

6. AP is commonly detected with the NBT/BCIP substrate.