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Star Republic: Guide for Biologists

Freezing virus

Many animal viruses can be stored by flash freeze the virus-containing supernatant in a dry-ice bath or liquid nitrogen, and stored at -70 C or in liquid nitrogen.

Viruses in supernatant or tissues can also be preserved by freeze-dry and stored at -70 C or in liquid nitrogen.

To recover frozen viruses, thaw quickly in 37 C. Avoid repeated freeze-and-thaw, some viruses can only be thawed once without destroying infectivity.