Virtualab Protocols (Chang Bioscience)
Sf9 and Sf21 cells can be grown as monolayer cultures using flasks
or tissue culture plates. Cells can be detached by pipetting the medium
across the monolayer. No Trypsin-EDTA is necessary. These cells can be grown
at room temperature or in a 27 °C incubator. They do not require CO2
Sf9 and Sf21 cells can also be grown in suspension in a shaker or spinner.
Seed cells at 0.5-1x 106 cells/ml in a shaker
or a roller bottle. Cells transfered from monolayer to suspension may not
grow well initially. Speed of shaking or spinning should increase gradually
to adapt cells in the suspension culture.