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Star Republic: Guide for Biologists

Storage of baculovirus stocks

1. Harvest the supernatant contianing the baculovirus. Centrifuge down cells and remove supernatnant containing the virus to fresh tubes.

2. For short term storage, store at 4 C in dark.

3. For long term storage, aliquot, and freeze at - 80 C.

4. If a serum-free medium is used, add BSA to final concentration of 0.1-1%. If desired, virus can also be filtered through a 0.2 mm, low-protein binding filter.