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Star Republic: Guide for Biologists

Staining of proteins ---SYPRO

1.

Dye Detection limits
SYPRO Ruby 1-2 ng/band, comparable to silver staining
SYPRO Orange/Red/Tangerine 4-8 ng/band, higher than Coomassie blue staining

2. Gels and membranes may need to be fixed first (e.g., 7% acetic acid/10% methanol for 10 minutes).

3. Stain in the staining solution for 15 minutes.

4. Rinse 2-3 times with fixing solution or deionized water.

5. Visulize the bands under UV or blue/blue-green light.