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Star Republic: Guide for Biologists

Western --- stripping

Edited by Chang Zhu
1. After ECL detection, wash the membrane twice for 10 minutes in PBS/0.05% Tween 20.

2. Strip in 65 mM Tris-HCl pH 6.7, 100 mM beta-mercaptoethanol, 2% SDS.

3. In a closed container, incubate at 50-70 C for 30 minutes.

4. Wash the membrane 3 times (10 minutes each) in a large volume of PBS/0.05% Tween 20 at room temperature.

5. Block and hybridize or wrap the membrane with Saran wrap and store frozen. Don't let the membrane dry completely.