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Star Republic: Guide for Biologists

PAGE --- recipe calculator

Tris-glycine SDS-Polyacrylamide Gel (SDS-PAGE Calculator)
Stacking Separating
Gel percentage (%) Gel percentage (%)
30% Polyacrylamide (mL) 30% Polyacrylamide (mL)
1M Tris(pH6.8)(mL) 1.5M Tris(pH8.8)(mL)
10% Ammonium persulfate (mL) 10% Ammonium persulfate (mL)
10% SDS (mL) 10% SDS (mL)
TEMED (mL) TEMED (mL)
H2O (mL) H2O (mL)
Total volume (mL) Total volume (mL)

Click here if polyacrylamide stock is 40%.

Click here for native PAGE.

See BioToolKit for more calculators.