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Star Republic: Guide for Biologists

Protein precipitation --- Trichloroacetic Acid (TCA)

1. Add equal volume of 40% TCA.

2. Precipiate at 4 C for 1 hour.

3. Centrifuge for 10 minutes at 4C and carefully remove and save supernatant.

4. Wash pellet gently with cold 100% acetone (-20 C) four times.

5. Dry sample briefly under vacuum and store sealed at -20 C.