Science Gateway > Protocols > Cell Biology Protocols - Table of Contents

Pubplus.org - a PubMed search engine
Star Republic: Guide for Biologists

Protein precipitation --- acetone

1. Add acetone to the final concentration of 80%.

2. Precipiate at -20 C overnight or in dry ice for 2-3 hours.

3. Centrifuge for 10 minutes at 4C and carefully remove supernatant.

4. Wash pellet gently with cold 100% acetone (-20 C) twice.

5. Dry sample briefly under vacuum and store sealed at -20 C.