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Star Republic: Guide for Biologists

Staining of proteins --- quick Coomassie blue

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1. Run a protein polyacrylamide gel.

2. Submerge the gel in the Commassie blue stain solution in a box with lid (e.g, an empty pipet tip box). Microwave on high for 1 min, then shake 10-20 min.

Commassie blue stain solution
Commassie Blue 600 mg
Water 260 ml
Methanol 240 ml
Acetic Acid 100 ml

Coomassie blue stain solution can be reused for serveral times.

3. Destaining in several changes of de-staining solution until protein bands are clear. Microwave on high for 1 min and shake until the bands emerge clearly from the background. Put a small sponge in the destaining solution to absorb coomassie blue dye particles.

De-staining solution
Water 1200 ml
Methanol 800 ml
Acetic Acid 200 ml

4. Gel can be photographed under white light, stored in the destaining solution, or dried.