Science Gateway > Protocols > Cell Biology Protocols - Table of Contents
Star Republic: Guide for Biologists

Equipments and Reagents
1 ml HiTrap NHS-activated column
1 ml HiTrap NHS-activated column Cat. No. 17-0716-01 (Amersham)
1,4-Diazabicyclo (2,2,2)-octane
1,4-Diazabicyclo (2,2,2)-octane Cat. No. D2 780-2 (Aldrich)
1,4-diphenylene-diisothiocyanate
1,4-diphenylene-diisothiocyanate Cat. No. 25 855-5 (Aldrich)
1-Butanol
1-Butanol Cat. No. 27 067-9 (Aldrich)
2-methylbutane
2-methylbutane Cat. No. M3 263-1 (Aldrich)
2X MEM
2X MEM Cat. No. 11 935 049 (Invitrogen)
3,3',5,5'-tetramethylbenzidine
3,3',5,5'-tetramethylbenzidine Cat. No. 86 033-6 (Aldrich)
3-Aminopropyl(diethoxy)methylsilane
3-Aminopropyl(diethoxy)methylsilane Cat. No. 09 309 (Fluka)
3-cyclohexylamino-1-propanesulfonic
3-cyclohexylamino-1-propanesulfonic Cat. No. 16 376-7 (Aldrich)
96 deep well plate
96 deep well plate Cat. No. 278 906 (Nunc)
96 pin replicating block
96 pin replicating block Cat. No. VP 404 (V&P Scientific)
96-well PCR plate
96-well PCR plate Cat. No. MPX-0296 (Phenix Research Products)
96-well sealing mat
Pierceable sealing mat for 96-well Cat. No. SMX-PCR96 (Phenix Research Products)
Non-pierceable sealing mat for 96-well Cat. No. MPCS-3510 (Phenix Research Products)
Abie
Abie Pro 3.0 for Windows/NT Cat. No. A02WI64 (Chang Bioscience)
Abie Pro 3.0 for Mac OSX Cat. No. A02MA65 (Chang Bioscience)
Acetic acid
Glacial acetic acid Cat. No. 53 702-0 (Aldrich)
Acetone
Acetone Cat. No. VW0370-4 (VWR)
Acetonitrile
Acetonitrile Cat. No. 34 896 (Aldrich)
Acetyl-CoA
Acetyl-CoA Cat. No. 101 893 (Roche)
Acrylamide
Acrylamide Cat. No. IC814 320 (ICN Biomedicals)
Adhesive plate sealer
Pierceable sealing mat for 96-well Cat. No. SMX-PCR96 (Phenix Research Products)
Non-pierceable sealing mat for 96-well Cat. No. MPCS-3510 (Phenix Research Products)
Agar
Agar Cat. No. IC10 026 280 (ICN Biomedicals)
Agarose
Agarose Cat. No. 2090 (EM Science)
alpha-factor
Alpha-factor mating pheromone Cat. No. Y1001 (Zymo Research)
Aluminum sulphate
Aluminum sulphate Cat. No. AX0746-1 (EM Science)
Amido Black
Amido Black Cat. No. 9012 (Helena)
aminoallyl-dUTP
aminoallyl-dUTP Cat. No. 8438 (Ambion)
Ammonium acetate
Ammonium acetate Cat. No. 2145 (EM Science)
Ammonium hydroxide
Ammonium hydroxide Cat. No. 22 122-8 (Aldrich)
Ammonium oxalate
Ammonium oxalate Cat. No. 37 974-3 (Aldrich)
Ammonium persulfate
Ammonium persulfate Cat. No. 21 558-9 (Aldrich)
Ammonium sulphate
Ammonium sulphate Cat. No. IC808 211 (ICN Biomedicals)
Ampicillin
Ampicillin Cat. No. 11593-019 (Invitrogen)
AMV reverse transcriptase
AMV reverse transcriptase Cat. No. 1 495 062 (Roche)
Amylose resin
Amylose resin Cat. No. E8021S (BioLabs)
anti-BrdU antibody
Mouse monoclone anti-BrdU Cat. No. 05-633 (Upstate)
anti-BrdU-FITC
anti-BrdU-FITC Cat. No. 1 202 693 (Roche)
anti-BrdU-POD
Anti-Bromodeoxyuridine peroxidase Cat. No. 1 585 860 (Roche)
anti-mouse IgG-FTIC
anti-mouse IgG-FTIC Cat. No. 100 843 (Roche)
anti-mouse IgG-horseradish peroxidase
Anti-mouse IgG-POD Cat. No. 605 30 (Roche)
Antifoam A
Antifoam A Cat. No. A6457 (Sigma)
Aphidicolin
Aphidicolin Cat. No. CC101-001 (BIOMOL research laboratories)
Apple juice
Apple juice (Supermarkets)
Aprotinin
Aprotinin Cat. No. IC19 038 210 (ICN Biomedicals)
Ascorbic acid
Ascorbic acid Cat. No. A9 290-2 (Aldrich)
ATCC mycoplasma PCR kit
ATCC mycoplasma PCR kit Cat. No. 90-1001K (ATCC)
B-M Cyclin
B-M Cyclin Cat. No. 799 050 (Roche)
Bacillus stearothermophilus
Spore test Cat. No. EMS-012 (Sterilizers)
Balb/c
Mouse strains (Jackson Laboratory)
Bath Clear
Bath Clear Cat. No. 13-641 334 (Fisher Scientific)
Bath Clear Algicide
Bath Clear Algicide Cat. No. CP-641 337 (Fisher Scientific)
BCIP
NBT/BCIP Cat. No. 18 280-016 (Invitrogen)
Benzyl benzoate
Benzyl benzoate Cat. No. B1,770-0 (Aldrich)
beta-mercaptoethanol
2-mercaptoethanol Cat. No. 6010 (EM Science)
Big dye terminator
Big dye terminator Cat. No. P/N 4 303 149 (PE Applied Biosystems)
BioRad Protein Assay Kit
BioRad Protein Assay Kit Cat. No. 500-0001 (BioRad)
Biotin-16-dUTP
Biotin-16-dUTP Cat. No. 1 093 070 (Roche)
biotin-dATP
biotin-dATP Cat. No. 19 524-016 (Invitrogen)
BioToolKit
BioToolKit 160 for Windows/NT Cat. No. B02WI60 (Chang Bioscience)
BioToolKit 160 for Mac OSX Cat. No. B02MA61 (Chang Bioscience)
Blue dextran
Blue dextran Cat. No. D5751 (Sigma)
Bluing reagent
Bluing reagent Cat. No. 65 354-85 (EM Science)
Boric acid
Boric acid Cat. No. 2710 (EM Science)
BrdU
BrdU Cat. No. 280 879 (Roche)
Bromocresol purple
Bromocresol purple Cat. No. 11 437-5 (Aldrich)
Bromophenol blue
Bromophenol blue Cat. No. 11 439-1 (Aldrich)
BSA
BSA Cat. No. 100 062 (Roche)
C. Therm. Polymerase one-step RT-PCR
C. Therm. Polymerase one-step RT-PCR Cat. No. 2 016 338 (Roche)
Cacodylate
Cacodylate Cat. No. C 0250 (Aldrich)
Calcium chloride
Calcium chloride Cat. No. 3000 (EM Science)
Canada balsam
Canada balsam Cat. No. B36 003-26 (EM Science)
Carbencillin
Carbencillin Cat. No. 10177-012 (Invitrogen)
Carboxyfluorescein succinimidyl ester
Carboxyfluorescein succinimidyl ester Cat. No. C-1311 (Molecular Probes)
CDTA
CDTA (1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid) Cat. No. 12 581-4 (Aldrich)
Cell Proliferation CellTiter Kit
Cell Proliferation CellTiter Kit Cat. No. G4000 (Promega)
Cell Proliferation ELISA Kit
Cell Proliferation ELISA Kit Cat. No. 1 669 915 (Roche)
Cell scraper
Cell scraper Cat. No. 3010 (Corning)
Cesium chloride
Cesium chloride Cat. No. 3030 (EM Science)
Chamber slides
Chamber slides Cat. No. 154 453 (Nunc)
CHAPS
CHAPS Cat. No. 810 118 (Roche)
Chickens
Live chickens (Commercial chicken farms)
Chloramine T
Chloramine T Cat. No. 40 286-9 (Aldrich)
Chloramphenicol
Chloramphenicol Cat. No. 634 433 (Roche)
Chloroform
Chloroform Cat. No. 3150 (EM Science)
CIP
Calf intestinal alkaline phosphatase Cat. No. M0290S (BioLabs)
Citric acid
Citric acid Cat. No. 27 488 (Aldrich)
Clear Bath Algae Inhibitor
Clear Bath Algae Inhibitor Cat. No. 1545920 (Spectrum Medical Industrial)
Cloning cylinders
Cloning cylinders Cat. No. F37 847-7000 (Bel-Art)
CNBr-activated Sepharose 4B beads
CNBr-activated Sepharose 4B beads Cat. No. 17-0430-01 (Amersham)
Cobalt chloride
Cobalt chloride Cat. No. 25 559-9 (Aldrich)
Colcemid
Colcemid Cat. No. 9311 (Irvine scientific)
Colchicine
Colchicine Cat. No. 00 345-500 (Polysciences)
Coomassie Blue
Coomassie brilliant blue G-250 Cat. No. 3300 (EM Science)
Cover slip
Cover slip Cat. No. 48393-026 (VWR)
CPRG
CPRG Cat. No. 884 308 (Roche)
Cryovials
Sterile 2.0 ml conical scrwcap tubes Cat. No. SCS-020S (Phenix Research Products)
Crystal violet
Crystal violet Cat. No. 22 928-8 (Aldrich)
Cyro freezing container
Cyro freezing container Cat. No. 5100-0001 (Nunc)
Cytocentrifuge
Cyto-Tek centrifuge 110 V Cat. No. 27 270 (Ted Pella)
Cyto-Tek centrifuge 220 V Cat. No. 27 270-220 (Ted Pella)
Cytocentrifuge cuvette
1 ml fluid chamber Cat. No. 27 270-1 (Ted Pella)
DAPI
DAPI Cat. No. 236 276 (Roche)
dATP
dATP Cat. No. 1 934 511 (Roche)
dCTP
dCTP Cat. No. 1 934 520 (Roche)
DEPC
Diethyl pyrocarbonate Cat. No. 15,922-0 (Aldrich)
DFM
N,N-Dimethylformamide Cat. No. 27 054-7 (Aldrich)
dGTP
dGTP Cat. No. 1 934 538 (Roche)
Dialysis tubing clip
Dialysis tubing clip Cat. No. 25 224-136 (VWR)
Diaminobenzidine
Diaminobenzidine (DAB) Cat. No. D1 238-4 (Aldrich)
dibasic sodium phosphate
dibasic sodium phosphate Cat. No. 0 192 (Canemco)
Dibutylphthalate
Dibutylphthalate Cat. No. 15 243-9 (Aldrich)
Diethanolamine
Diethanolamine Cat. No. D8 330-3 (Aldrich)
Digoxigenin-11-dUTP
Digoxigenin-11-dUTP Cat. No. 1 093 088 (Roche)
Digoxigenin-dUTP
Digoxigenin-dUTP Cat. No. 2 093 088 (Roche)
Dimethyl formamide
Dimethyl formamide Cat. No. 27 054-7 (Aldrich)
Dimethyl pimelimidate
Dimethyl pimelimidate Cat. No. CLI-103 (ALCHEMY)
Dimethyl pimelimidate Cat. No. D8388 (Sigma)
Diphenylamine
Diphenylamine Cat. No. 11 276-3 (Aldrich)
Dipper papers
Assay mats Cat. No. 56 616-009 (VWR)
dITP
dITP Cat. No. 1 051 474 (Roche)
DMSO
DMSO Cat. No. 47 157-7 (Aldrich)
Dnase I
Dnase I Cat. No. 2222 (Ambion)
dNTP
dNTP Cat. No. N0447S (BioLabs)
DOTAP
DOTAP Cat. No. 1 811 177 (Roche)
DpnI
DpnI Cat. No. R0176S (BioLabs)
Dry yeast
Dry yeast Cat. No. WW-17-3235 (Carolina Biological)
DTT
DTT Cat. No. 100 032 (Roche)
dTTP
dTTP Cat. No. 1 934 546 (Roche)
dUTP
dUTP Cat. No. 1 934 554 (Roche)
DyNAzyme EXT DNA Polymerase
DyNAzyme EXT DNA Polymerase Cat. No. F-505S (Finnzymes)
Ear punch
Ear punch Cat. No. 01-337B (Fisher Scientific)
ECL
ECL Cat. No. RPN1237 (Amersham)
EDTA
EDTA Cat. No. 4005 (EM Science)
Electroporation cuvette
1 mm gap electroporation cuvette Cat. No. 47727-640 (VWR)
2 mm gap electroporation cuvette Cat. No. 47727-642 (VWR)
4 mm gap electroporation cuvette Cat. No. 47727-644 (VWR)
ELISA plate
ELISA plate Cat. No. 12-565-136 (Nunc)
Eosin Y
Eosin Y Cat. No. EX0036-4 (EM Science)
Ethanolamine
Ethanolamine Cat. No. 41,100-0 (Aldrich)
Ethidium bromide
Ethidium bromide Cat. No. 16 053-9 (Aldrich)
Exonuclease I
Exonuclease I Cat. No. M0293S (BioLabs)
Faceshield
Faceshield Cat. No. 33 007-107 (VWR)
Factor Xa
Factor Xa Cat. No. 1 179 888 (Roche)
Ficoll
Ficoll Cat. No. 341 691CB (Voigt Global Distribution)
FITC
Fluorescein isothiocyanate Cat. No. F-143 (Molecular Probes)
FITC-Avidin
FITC-Avidin Cat. No. 43-4411 (Zymed Laboratories)
Fluor-coated TLC plate
Fluor-coated TLC plate Cat. No. 10 110 (Ambion)
Fluorescein isothiocyanate
Fluorescein isothiocyanate Cat. No. F-143 (Molecular Probes)
Fluorescein-12-dUTP
Fluorescein-12-dUTP Cat. No. 1 373 242 (Roche)
Flynap
Flynap Cat. No. WW-17-3010 (Carolina Biological)
Formaldehyde
Formaldehyde Cat. No. 53 399-8 (Aldrich)
Formalin
Formalin Neutral Buffered Cat. No. 65 346-85 (EM Science)
Formamide
Formamide Cat. No. 47 670 (Aldrich)
FuGene 6
FuGene 6 Cat. No. 1 815 091 (Roche)
Gelvatol
Polyvinyl alcohol Cat. No. 9 002 895-1 (Chemistry Store)
Genius Kit
Genius Kit Cat. No. 1 093 657 (Roche)
Giemsa
Giemsa Cat. No. R03 055-74 (EM Science)
Gloves
Bio-Flex premium gloves Cat. No. GP-999 (Phenix Research Products)
Glutaraldehyde
Glutaraldehyde Cat. No. 34 085-5 (Aldrich)
Glycine
Glycine Cat. No. 4840 (EM Science)
Godlist
Godlist Manager 1.0 for Windows/NT Cat. No. G02WI8 (Chang Bioscience)
Godlist Manager 1.0 for Mac OSX Cat. No. G02MA9 (Chang Bioscience)
Guanidine
Guanidine hydrochloride Cat. No. 5010 (EM Science)
Gum acacia
Gum acacia Cat. No. 26 077-0 (Aldrich)
Heatblock
Dry block heater 120 V Cat. No. 13 259-030 (VWR)
Hematoxylin
Hematoxylin Harris Cat. No. 638-85 (EM Science)
HEPES
HEPES Cat. No. 737 151 (Roche)
HisTrap column
HisTrap column Cat. No. 17-1880-01 (Amersham)
Hoechst
Hoechst 33258 Cat. No. H-21491 (Molecular Probes)
Hydrogen peroxide
Hydrogen peroxide Cat. No. 21,676-3 (Aldrich)
Hydroxylamine
Hydroxylamine Cat. No. 46 780-4 (Aldrich)
Hydroxyurea
Hydroxyurea Cat. No. 11 520-7 (Aldrich)
Hygromycin B
Hygromycin B Cat. No. 843 555 (Roche)
Hyperscreen
Hyperscreen (18x24 cm) Cat. No. RPN1662 (Amersham)
IDOD-Beads
IDOD-Beads Cat. No. 28 665 (Pierce)
IgY HiTrap column
IgY HiTrap column Cat. No. 17-5111-01 (Amersham)
Imidazole
Imidazole Cat. No. 5710 (EM Science)
Instant Drosophila medium
Instant Drosophila medium (Nebraska Scientific)
Instant Drosophila medium Cat. No. WW-17-3200 (Carolina Biological)
IPTG
IPTG Cat. No. 15 529-019 (Invitrogen)
IsoStrip Antibody Isotyping
IsoStrip Antibody Isotyping Cat. No. 1 493 027 (Roche)
Kanamycin
Kanamycin Cat. No. 11 815-024 (Invitrogen)
keyhole limpet hemocyanin
keyhole limpet hemocyanin Cat. No. 77 649 (Pierce)
Klenow
Klenow fragment of DNA polymerase I Cat. No. 600 071 (Stratagene)
Kodak BioMax MS
Kodak BioMax MS (5x7 in) Cat. No. V8 142 358 (Amersham)
Kodak BioMax TranScreen
Kodak BioMax TranScreen (35x43 cm) Cat. No. V8 660 789 (Amersham)
Lithium acetate
Lithium acetate Cat. No. 57 799-2 (Aldrich)
Lithium chloride
Lithium chloride Cat. No. 5910 (EM Science)
Luciferase assay reagent
Luciferase reporter gene assay Cat. No. 1 669 893 (Boehringer Mannheim)
Luminometer
Luminometer Cat. No. 551 280 (BD Biosciences)
Lyticase
Lyticase Cat. No. L8137 (Sigma)
Lyzozyme
Lyzozyme Cat. No. 1 243 004 (Roche)
m-maleimidobenzoyl-N-hydroxysuccinimide ester
m-maleimidobenzoyl-N-hydroxysuccinimide ester Cat. No. 442 625 (Calbiochem)
Maltose
Maltose Cat. No. 11 256-9 (Aldrich)
MEGAscript II Kit
MEGAscript SP6 Kit Cat. No. 1330 (Ambion)
MEGAscript T7 Kit Cat. No. 1334 (Ambion)
MEGAscript T3 Kit Cat. No. 1338 (Ambion)
Methanol
Methanol Cat. No. VW4325-4 (VWR)
Methylene blue
Methylene blue Cat. No. US19 220 (Amersham)
Microcon-10
Microcon-10 Cat. No. AMI-42 406 (Millipore)
microcon-100
microcon-100 Cat. No. AMI-42 412 (Millipore)
microcon-3
microcon-3 Cat. No. AMI-42 403 (Millipore)
microcon-30
microcon-30 Cat. No. AMI-42 409 (Millipore)
microcon-50
microcon-50 Cat. No. AMI-42 415 (Millipore)
MicroHelper
MicroHelper 1.02 for Windows/NT Cat. No. M02WI1 (Chang Bioscience)
MicroHelper 1.02 for Mac OSX Cat. No. M02MA2 (Chang Bioscience)
Minelute column
Minelute column (Qiagen)
Molasses
Molasses Cat. No. WW-17-3232 (Carolina Biological)
monobasic sodium phosphate
monobasic sodium phosphate Cat. No. 567 545CB (Vogit Global Distribution)
MOPS
MOPS Cat. No. 6350 (EM Science)
Mowiol
Mowiol Cat. No. 32 459-0 (Aldrich)
MUG
MUG Cat. No. M-1489 (Molecular probes)
N-Lauroylsarcosine
N-Lauroylsarcosine Cat. No. 61 739 (Aldrich)
NBT
NBT/BCIP Cat. No. 18 280-016 (Invitrogen)
NBT/BCIP
NBT/BCIP Cat. No. 18 280-016 (Invitrogen)
NHS-Biotin
NHS-Biotin Cat. No. UP39044A (Interchim)
Nickel chloride
Nickel chloride Cat. No. 20 386-6 (Aldrich)
Nitrocellulose filter
Optitran BA-S 85 discs Cat. No. 10 439 148 (S&S)
Nitrocellulose membrane
Nitrocellulose membrane Cat. No. LC2000 (Invitrogen)
Nocodazole
Nocodazole Cat. No. 74 151 (Aldrich)
Nuclear fast red
Nuclear fast red Cat. No. 7602-8 (EM Science)
Oligo-dT
p(dT)15 Cat. No. 814 270 (Roche)
p(dT)10 Cat. No. 814 261 (Roche)
Oligo-dT cellulose
Oligo-dT cellulose Cat. No. 10 020 (Ambion)
Oligotex mRNA Midi Kit
Oligotex mRNA Midi Kit Cat. No. 70 042 (Qiagen)
ONPG
ONPG (o-nitrophenyl-beta-D-galactopyranoside) Cat. No. 2 360 (BioVectra)
Orange G
Orange G Cat. No. 86 128-6 (Aldrich)
Oxoid Nutrient Broth #2
Oxoid Nutrient Broth #2 (Unipath Company, Oxoid Division, P. O. Box 691, NY 13669, 800 - 567-8378 )
p-phenylenediamine
p-phenylenediamine Cat. No. 007 150 525 (VWR)
Paraformaldehyde
Paraformaldehyde Cat. No. 15 812-7 (Aldrich)
PD-10
Disposable PD10 desalting columns Cat. No. 17-0851-01 (Amersham)
PEG 4000
PEG 4000 Cat. No. 81 240 (Aldrich)
PEG 8000
Polyethylene glycol 8000 Cat. No. 6510 (EM Science)
Pfu DNA polymerase
Pfu DNA polymerase Cat. No. 600 135 (Stratagene)
Pfu Turbo DNA polymerase Cat. No. 600 250 (Stratagene)
pH standard buffers
pH 7 buffer Cat. No. 34 170-130 (VWR)
pH 4 buffer Cat. No. 34 170-127 (VWR)
pH 10 buffer Cat. No. 34 170-133 (VWR)
Phenazine methosulfate
Phenazine methosulfate Cat. No. P1 340-1 (Aldrich)
Phenol
Phenol Cat. No. 6610 (EM Science)
Phenol red
Phenol red Cat. No. 11 452-9 (Aldrich)
Pico green
PicoGreen dsDNA quantitation kit Cat. No. P-11495 (Molecular probes)
PIPES
Piperazine-N,N'-bis(2-Ethanesulfonic acid), sodium salt Cat. No. 6910 (EM Science)
PMSF
Phenylmethyl sulfonyl fluoride Cat. No. 7710 (EM Science)
Poly-L-Lysine
Poly-L-Lysine Cat. No. P2980 (Sigma)
PolyA tailing
PolyA tailing Cat. No. 1350 (Ambion)
Polyethylene glycol
Polyethylene glycol 8001 Cat. No. 6511 (EM Science)
Ponceau S
Ponceau S Cat. No. 14 119-4 (Aldrich)
Potassium acetate
Potassium acetate Cat. No. 23 649-7 (Aldrich)
Potassium iodide
Potassium iodide Cat. No. 20 410-2 (Aldrich)
Primo
Primo Pro 3.0 for Windows/NT Cat. No. P02WI68 (Chang Bioscience)
Primo Pro 3.0 for Mac OSX Cat. No. P02MA69 (Chang Bioscience)
Propidium Iodide
Propidium Iodide Cat. No. 1 348 639 (Roche)
Propyl gallate
Propyl gallate Cat. No. P5 330-6 (Aldrich)
Protease inhibitor cocktail
Protease inhibitor cocktail Cat. No. 1 206 893 (Roche)
Protease inhibitor set
Protease inhibitor set Cat. No. 2 206 893 (Roche)
Protein A
Protein A-agarose Cat. No. 1 719 408 (Roche)
Protein A-agarose
Protein A-agarose Cat. No. 2 719 408 (Roche)
Protein A/G
Protein A/G Cat. No. sc-2003 (Santa Cruz Biotechnology)
Protein G
Protein G-agarose Cat. No. 1 719 416 (Roche)
Protein G-agarose
Protein G-agarose Cat. No. 2 719 416 (Roche)
Proteinase inhibitor cocktail
Proteinase inhibitor cocktail Cat. No. 1 206 893 (Roche)
Proteinase K
Proteinase K Cat. No. 161 519 (Roche)
PVDF membrane
PVDF membrane Cat. No. 28 165-521 (S&S)
Pyridine
Pyridine Cat. No. 27 097-0 (Aldrich)
Qiagen plasmid midi prep
Qiagen plasmid midi prep Cat. No. 12 174 (Qiagen)
Qiagen-tip
Qiagen-tip 20, mini prep Cat. No. 12 123 (Qiagen)
Qiagen-tip 100, midi prep Cat. No. 12 143 (Qiagen)
Qiagen-tip 500, maxi prep Cat. No. 12 162 (Qiagen)
Quartz cuvette
UV Silica Cuvette 10 mm Pathlength Cat. No. BK580012 (VWR)
Quick ligation kit
Quick ligation kit Cat. No. M2200S (BioLabs)
Rabbit Reticulocyte Lysate
Rabbit Reticulocyte Lysate Cat. No. L4960 (Promega)
Raffubise oebtagtdrate
Raffubise oebtagtdrate Cat. No. 20 667-9 (Aldrich)
Rhodamine-phalloidin
Rhodamine-phalloidin Cat. No. R-415 (Molecular probes)
Ribonuclease
RNase (DNase-free) Cat. No. 1 119 915 (Roche)
Ribonuclease inhibitor
RNase inhibitor Cat. No. 799 025 (Roche)
RNase A
RNase A Cat. No. 12 091-021 (Invitrogen)
Rnase Inhibitor
RNase inhibitor Cat. No. 799 025 (Roche)
RNase T1
RNase T1 Cat. No. 2280 (Ambion)
RNasin Inhibitor
RNase inhibitor Cat. No. 799 025 (Roche)
RNeasy Kit
RNeasy mini Kit Cat. No. 74 104 (Qiagen)
RNeasy midi Kit Cat. No. 74 154 (Qiagen)
RNeasy maxi Kit Cat. No. 74 162 (Qiagen)
Salmon sperm dna
Salmon sperm dna Cat. No. 15 632-011 (Invitrogen)
Salmonella strains
Salmonella strains (BN Ames, University of California, Berkley)
Scintillation cocktail
Econo safe liquid scintillation fluid Cat. No. 111 175 (Atlantic nuclear)
Scintillation fluid
Econo safe liquid scintillation fluid Cat. No. 111 175 (Atlantic nuclear)
Sephadex G10
Sephadex G10 Cat. No. 17-0010-01 (Amersham)
Sephadex G25
Sephadex G25 DNA grade Cat. No. 17-0572-01 (Amersham)
Sephadex G50
Sephadex G50 Cat. No. 17-0043-01 (Amersham)
Sequenase kit
DNA sequencing kit Cat. No. 70 770 (USB)
Shrimp alkaline phosphatase
Shrimp alkaline phosphatase Cat. No. 1 758 250 (Roche)
Silica beads
Silica beads Cat. No. S-5631 (Sigma)
Silica-gel TLC plate
Silica-gel TLC plate Cat. No. 5715-7 (EM Science)
Slide
Slide Cat. No. 48 300-047 (VWR)
Slide-A-Lyzer
3.5K dialysis kit Cat. No. PI66 335 (VWR)
10K dialysis kit Cat. No. PI66408 (VWR)
Sodium acetate
Sodium acetate Cat. No. 7510 (EM Science)
Sodium azide
Sodium azide Cat. No. 19 993-1 (Aldrich)
Sodium borate
Sodium borate Cat. No. 22,994-6 (Aldrich)
Sodium cacodylate
Sodium cacodylate Cat. No. 19 (Canemco)
Sodium deoxycholate
Sodium deoxycholate Cat. No. 264 101 (Calbiochem)
Sodium nitrite
Sodium nitrite Cat. No. 52 437-9 (Aldrich)
Sodium vanadate
Sodium vanadate Cat. No. 72 060 (Aldrich)
Sorbitol
Sorbitol Cat. No. 30 953-2 (Aldrich)
Streptavidin-fluorescein
Streptavidin-fluorescein Cat. No. NEL720 (Perkin Elmer)
Streptavidin/HRP
Streptavidin/HRP Cat. No. 3 034 755 (Roche)
Streptomycin
Streptomycin Cat. No. 11 860-038 (Invitrogen)
Suc-LLVY-AMC
Suc-LLVY-AMC Cat. No. P-802 (BioMol Research Laboratories)
Sulforhodamine B
Sulforhodamine B Cat. No. S-1307 (Molecular probes)
SYBR green
SYBR Green I nucleic acid gel staining Cat. No. S-7585 (Molecular probes)
SYBR Green II RNA gel staining Cat. No. S-7586 (Molecular probes)
T4 DNA ligase
T4 DNA ligase Cat. No. M0202S (BioLabs)
T4 ligase
T4 ligase Cat. No. M0202S (BioLabs)
T4 polynuclotide kinase
T4 polynuclotide kinase Cat. No. M0201S (BioLabs)
TaKaRa Ex Taq
TaKaRa Ex Taq Cat. No. TAK RR001A (Panvera)
TALON
TALON superflow metal affinity resin Cat. No. 8908-1 (BD Biosciences Clontech)
Taq DNA polymerase
Taq DNA polymerase Cat. No. 600 131 (Stratagene)
TCA
Trichloroacetic acid Cat. No. 91 232 (Aldrich)
Tegosept
Tegosept Cat. No. BA-87-6161 (Carolina Biological)
TEMED
TEMED Cat. No. 100 139 (Roche)
Terminal transferase
Terminal transferase Cat. No. 220 582 (Roche)
Tetracycline
Tetracycline Cat. No. Q100-19 (Invitrogen)
Texas red-avidin
Texas red-avidin Cat. No. A-2016 (Vector Labs)
Texas-red
Texas-red Cat. No. 016 070 084 (Jackson Immuno)
Thrombin
Thrombin Cat. No. 602 400 (Roche)
Thymidine
Thymidine Cat. No. 85 500-6 (Aldrich)
Thymol
Thymol Cat. No. 11 209-7 (Aldrich)
TLC developing chamber
TLC developing chamber Cat. No. 276 860 (Wheaton Science Products)
TNT coupled transcription and translation kit
TNT PCR Quick for PCR DNA Cat. No. L5540 (Promega)
Tris
2-Amino-2-(hydroxymethyl)-1,3-propanediol;Tris(hydroxy-methyl)aminomethane Cat. No. 9210 (EM Science)
Trizol
Trizol Cat. No. 15 596-026 (Invitrogen)
Trypan blue
Trypan blue Cat. No. 30 264 (Aldrich)
Trypsin-EDTA
Trypsin-EDTA(0.025% Trypsin 0.01% EDTA) Cat. No. R-001-100 (Cascade Biologics)
Urea
Urea Cat. No. 9510 (EM Science)
UV box
Ultraviolet transilluminators (115V) Cat. No. 21 475-468 (VWR)
Ultraviolet transilluminators (230V) Cat. No. 21475-470 (VWR)
UV crosslinker
Stratalinker UV crosslinker 120V Cat. No. 400 071 (Stratagene)
UVP safety goggles
UVP safety goggles Cat. No. 33 002-078 (VWR)
Vacuum blotter
PosiBlot 30-30 Pressure Blotter 120V Cat. No. 400 331 (Stratagene)
VCSM13
Uni-ZAP XR Vector Kit Cat. No. 237 211 (Stratagene)
Vectamount
Vectamount permanent mounting medium Cat. No. H-5000 (Vector Labs)
Vectashield
Vectashield mounting medium Cat. No. H-1000 (Vector Labs)
Vectastain ABC kit
Vectastain ABC-AP kit (goat IgG) Cat. No. AK-5005 (Vector Labs)
Wallac microbeta scintillation counter
Wallac microbeta scintillation counter Cat. No. 1450-024 (Perkin Elmer)
Weigerts Iodine
Weigerts Iodine Cat. No. 12 092 (Newcomer Supply)
Well slide
Well slide Cat. No. 1519 (Erie)
Whatman 3MM paper
Whatman 3MM paper Cat. No. 3001-931 (Whatman)
Whatman DE81
DE81 DEAE Cellulose Cat. No. 3658-323 (Whatman)
Whatman filter
Whatman filter (Whatman)
Whatman GF/C glass-fiber disc
Whatman GF/C glass-fiber disc Cat. No. 1822-021 (Whatman)
Whatman P81 phosphocellulose filter
Whatman P81 phosphocellulose filter Cat. No. 3698-023 (Whatman)
X-gal
X-gal Cat. No. 15 520-034 (Invitrogen)
XL-1 blue
XL-1 blue Cat. No. 200 294 (Stratagene)
XL-1 blue (MRF) Cat. No. 200301 (Stratagene)
XTT-tetrazolium
XTT-tetrazolium Cat. No. XTT101 (Lemargo)
Xylene
Xylene Cat. No. 72 060-088 (VWR)
Xylene cyanol FF
Xylene cyanol FF Cat. No. 9710 (EM Science)
Xylene cyanole FF
Xylene cyanole FF Cat. No. 9711 (EM Science)
Xylol
Xylene Cat. No. 72 060-088 (VWR)
Zeocin
Zeocin Cat. No. R250-01 (Invitrogen)
Zwittergent
Zwittergent 3-08 detergent Cat. No. 693 019 (Calbiochem)
Zymolyase
Zymolyase Cat. No. E1003 (Zymo research)
Lyticase Cat. No. L8137 (Sigma)
Zymolase Cat. No. 32 093-2 (ICI)