1. Put a 0.5-1.5 cm mouse tail snip in a 1.5 ml eppendorf tube and store
frozen util digestion. Tails should never be left at room temperature.
2. To each tube, add 500 ml of tail prep solution:
3. Make sure the tails are submerged. Digest at 55 °C overnight.
4. Cool samples on the ice. Add 0.5 ml phenol, mix, and spin 12,000 rpm x 10 minutes at room
5. Transfer the aqueous phase to a new eppendorf tube. Add 0.5 ml
phenol:chloroform:isoamyl alchol (25:24:1), mix, and spin 12,000 rpm x 10 minutes at room
6. Repeat Step 5 with 0.5 ml chloroform.
7. Transfer the aqueous phase to a new eppendorf tube, add equal volume 95% ethanol.
Gently invert the tube several times to precipitate DNA.
8. Centrifuge 10-15 minutes at 12,000 rpm.
9. Remove the supernatant, wash pellet with cold 70% ethanol.
10. Dry the pellet. Don't over dry since genomic DNA may stick to the
eppendorf tube permanently. Resuspend pellet in 200-400 ml TE.