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Star Republic: Guide for Biologists

Sex of mouse embryo and cell line

1. A genomic PCR can be used to determine the sex of a mouse embryo.

2. Smac primers:

Forward: 5'-TGAAGCTTTGGCTTTGAG-3'
Reverse: 5'-CCGCTGCCAAATTCTTTGG-3'

3. PCR products in male: 290 bp (Smac on Y) and 330 bp (Smac on X). Females have only one product: 330 bp (Smac on X).

4. PCR program: 95 C for 30 sec, 58 C for 30 sec, 72 C for 30 sec, 35 cycles.