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Star Republic: Guide for Biologists

NIH Open Formula Rat and Mouse Diet 9% Fat Autoclavable (NIH-36)

NSN-8710-00-L63-8761

Ingredients Percentage by weight
Fish meal (60% protein) 9.00
Soybean meal (48.5% protein) 5.00
Alfalfa meal (17% protein) 2.00
Corn gluten meal (60% protein) 3.00
Ground whole soft wheat 40.65
Ground #2 yellow corn 15.00
Ground whole oats 5.00
Wheat middlings 10.00
Soy oil 6.20
Salt 0.50
Dicalcium phosphate 1.50
Ground limestone 0.50
NIH31 Vitamin Premix 0.25
NIH31 Mineral Premix 0.25
Choline CL-70% 0.15
Yeast-Brewers       1.00
  100.00

All ingredients shall be ground to pass through a U.S. Standard Screen No. 16 prior to mixing.

Vitamin Fortification per ton (2,000 lbs.) of finished product

Vitamin Amount   Source
A 22,000,000 IU Vitamin A palmitate or acetate
D3 3,800,000 IU D activated animal sterol
K 20 g. Menadione activity
dl Alpha-tocopheryl acetate 15 g.
Choline 700 g. Choline chloride
Folic acid 1 g.
Niacin 40 g.
d Pantothenic acid 25 g. d-Calcium pantothenate
Riboflavin supplement 5 g.
Thiamin 65 g. Thiamin mononitrate
B12 supplement 40,000 mcg.
Pyridoxine 5 g. Pyridoxine hydrochloride
Biotin 120 mg. d-Biotin

Mineral Fortification Per Ton (2,000) of Finished Product

Mineral Amount   Source
Cobalt 400 mg. Cobalt carbonate
Copper 4 g. Copper sulfate
Iron 60 g. Iron sulfate
Magnesium 400 g. Magnesium oxide
Manganese 100 g. Manganese oxide
Zinc 10 g. Zinc oxide
Iodine 1500 mg. Calcium iodate

These concentrations of vitamins and minerals shall be added to the ration via two separate (vitamin and mineral) premixes. For the mineral fortification, the actual amount of each element required is specified. Therefore, the contractor shall adjust the amount of each compound used in the premix according to its mineral concentration.

Nutrient Standards

Micro Analysis - The total calculated concentrations of nutrients in the ration from ingredients and from the fortifications at the time of manufacture should be as follows:

Crude protein % Minimum 18.0
Crude fat % Minimum 9.0
Crude fiber % Maximum 5.0
Ash % Maximum 8.0

Amino Acids
(% of total diet)
  Minimum
Arginine .90
Lysine .85
Methionine .35
Cystine .25
Tryptophan .20
Glycine .95
Histidine .38
Leucine 1.40
Isoleucine .95
Phenylalanine .85
Tyrosine .60
Threonine .65
Valine .90

Minerals   Minimum
Calcium % 1.15
Phosphorous % .85
Potassium % .75
Sodium % .30
Magnesium % .15
Iron PPM 345.00
Zinc PPM 40.00
Manganese PPM 140.00
Copper PPM 12.00
Cobalt PPM 0.70
Iodine PPM 1.80

Vitamins   Minimum
Vitamin A IU/g 20.0 (10)*
Vitamin D IU/g 4.0
Alpha-tocopherol PPM 45.0
Thiamin PPM 70.0
Riboflavin PPM 7.0
Niacin PPM 80.0
Pantothenic acid PPM 30.0
Choline PPM 1900.0
Pyridoxine PPM 10.0
Folic acid PPM 2.0
Biotin PPM .2
Vitamin B12 mcg/Kg 40.0
Vitamin K PPM 20.0

* TRUE VITAMIN A ACTIVITY BY HPLC METHOD