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Star Republic: Guide for Biologists

Routes of injection in mouse

ChangBioscience.com

Routes of injection

Sites

Needle

Volume

Intravenous (iv)

directly into the vascular system through a vein

Tail, warm with water or lamp.

27-30 g

< 0.2 ml

Intra-arterial (ia)

directly into the vascular system through an artery

Intraperitoneal (ip)

into the abdominal cavity

Lateral aspect of the lower left quadrant

23-27 g

< 2 ml

Subcutaneous (sc)

under the skin

Loose skin between the shoulder blades

25-27 g

< 2 ml

Intramuscular (im)

into a muscle

Back and hind leg muscles

26-30 g

< 0.05 ml

Intradermal (id)

between layers of skin