Endotoxin (pyrogen) may be decontaminated by heating at 250-260 °C in a
depyrogenation oven (Sheldon Manufacturing) for no less than 1 hour.
Lower temperature (180-200 °C) and longer time (3-4 hours) may also be
Acid or Base
Plastic and glassware may be decontaminated by soaking in strong
acid (e.g., 0.1 M hydrochloric acid) or base (e.g., 0.5 M NaOH).
Soak for 1 hour to overnight, and rinse thoroughly with
Columns may also be decontaminated using strong acid (e.g., 0.1-1 M hydrochloric acid; 60% ethanol, 0.1-1 M acetic acid) or base (e.g., 0.1-2 M sodium hydroxide).
Surfaces (e.g., tubing, rubber and metal), filtration columns and membranes may be decontaminated with PyroCLEAN (ALerCHEK, Cat. No. I30103).