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Star Republic: Guide for Biologists

Buffer exchange --- Slide-A-Lyzer dialysis cassettes

Virtualab Protocols (Chang Bioscience)
1. Select a Slide-A-Lyzer dialysis cassette with an appropriate molecular weight cutoff.

2. Inject sample into the Slide-A-Lyzer dialysis cassette using a syringe and a 18-gauge needle. Remove trapped air use the same syringe and needle.

3. Dialysis in at least 100X volume of the buffer the sample will be exchanged into. Stir slowly with a magnetic bar. Cool the buffer before hand and dialysis at 4 C if the sample is temperature sensitive.

4. Change the buffer every 2-4 hours for at least 3 times.

5. Carefully remove the sample out of the cassette with a clean syringe and needle.