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Star Republic: Guide for Biologists

PCR amplification of library insert

Edited by Chang Zhu
1. Phage DNA can be prepared as described in Phage clone --- pick clones for storage.

2. 1 ml of phage (108 pfu) or less may be PCR amplified using phage specific primers (e.g., gt11 forward and reverse) in a 25 ml PCR reaction.

See also PCR amplification of cDNA library inserts.