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Star Republic: Guide for Biologists

Phage clone --- storage

1. Phage clone and library may be stored at 4 C in 10 mM Tris-HCl pH7.5, 10 mM MgCl2, 50 mM NaCl.

2. Keep bacteria from growing by adding one drop of chloroform.

3. Seal tightly.

4. For long term storage, add 10% DMSO, store at -70 C.

5. A working stock diluted from the original stock is frequently stored at 4 C.

6. Titers of the working stock should be at least 106 pfu/ml.