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Star Republic: Guide for Biologists

Iodination protocol --- IODO-BEADS

IDOD-BEADS is from Pierce.

1. Volatile 125I2 may be produced. Work under a chemical hood. Proper protection is also needed when working with 125I.

2. Incubate one IDOD-Bead in 0.2 ml of 0.1 M Tris-HCl (pH 7) in a microfuge tube for 5 minutes at room temperature.

3. Add 0.2 mg of purified protein and 0.2 mCi of Na125I.

4. Incubate for 15 minutes at room temperature.

5. Remove the solution containing ioiodinated protein.

6. Separate by gel filtration.