1. Ethanol precipitation can be used to separate DNA from unincorporated nucleotides.
When working with radiolabeled probes, proper protection and waste disposal are required.
2. Add 0.5 volumes of 7.5 M ammonium acetate and 2 volumes of cold
ethanol (-20 °C), vortex, centrifuge at 12,000 rpm for 30 minutes.
3. Remove supernatant, add 3 volumes cold 70% ethanol (-20 °C),
centrifuge at 12,000 rpm for 10 minutes.
4. Remove supernatant, air dry, and resuspend the probe in a suitable