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Star Republic: Guide for Biologists

Labeling of DNA in agarose gel

1. Run 5 mg of DNA to be labeled on an agarose gel.

2. Visulize the DNA under a UV light, cut out a slice of the gel containing the target DNA. Keep the volume of gel slice minimum.

3. Add 1 ml ddH2O, melt the gel and denature DNA by boiling. Keep at 37 C.

4. Prepare a reaction mix without the template. See DNA random labeling with 32P-dCTP.

5. Add 10 ml to the reaction mix. Proceed as has been described in DNA random labeling with 32P-dCTP.