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Star Republic: Guide for Biologists

Electroporation --- parameters for different cells

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The parameters in the table below may be used as a starting point for optimization. 4 mm cuvettes are used for animal cells and 1 mm for others.

V (v)

C (mF)

Q (c)

R (W )

V/D (kv/cm)

BHK-21

400

960

0.4

1

Cos-7

350

500

0.2

0.9

ES

200

500

0.1

0.5

HL-60

350

960

0.3

0.9

Jurkat

250

960

0.2

0.6

PC12

200

800

0.2

0.5

U936

350

960

0.3

0.9

Aspergillus nidulans

500

25

400

5

E. Coli

1700

25000

200

17

Neurospora crassa

150

25000

600

1.5

Yeast

900

50

100

9