1. Place tissue specimens directly onto wet ice or into
10% neutral buffered formalin immediately after surgical removal or biopsy.
2. For sectioning, grossly process the tissue specimen into thin sections (< 3 mm) or
cut into small pieces. Uncut tissues can be preserved in formalin.
3. Place into a large container with at least 10 volumes of 10% neutral buffered
formalin and fix overnight.
Note: The routine fixative formalin preserves the tissue morphology,
it also cross-links RNA and DNA to protein. Although not impossible,
it will be less preferable for PCR or other molecular
applications. Ethanol fixation or snap freezing may be used instead.