Science Gateway > Protocols > Cell Biology Protocols - Table of Contents
Star Republic: Guide for Biologists

Paraffin embedding

1. Place fixed tissues in the tissue processor.

2. Process using the following or similar program.

Station

Solution

Concentration

Time(min)

Temp(C)

1

Ethanol

70%

30

2

Ethanol

80%

30

3

Ethanol

95%

45

4

Ethanol

95%

45

5

Ethanol

100%

45

6

Ethanol

100%

45

7

Xylenes

100%

45

8

Xylenes

100%

45

9

Paraffin

30

60

10

Paraffin

30

60

11

Paraffin

30

60

12

Paraffin

30

60

3. Embed in paraffin and block.

4. Cut sections at 5-8 ml thickness.

5. Paraffin blocks and sections may be stored at room temperature or below.