Science Gateway > Protocols > Cell Biology Protocols - Table of Contents

Pubplus.org - a PubMed search engine
Star Republic: Guide for Biologists

Mounting medium --- anti-fade

1. Dissolve p-phenylenediamine (10 mg) or n-propyl gallate (50 mg) in 1 ml of PBS or 0.1 M Tris-HCl pH 9.0. Keep wrapped in the dark.

2. Add 9 ml of glycerol. Mix by stirring. Keep wrapped in the dark.

3. (optional) Adjust pH to 8.0 using 0.1 M sodium carbonate buffer, pH 9.2. (mix 0.2 M sodium carbonate and 0.2 M sodium bicarbonate 1:11.5)

4. Store at -20 C in the dark.

Other antiquench agents:

  • 1,4-Diazabicyclo (2,2,2)-octane (DABCO) (~2%)
  • Ascorbic acid (vitamin C)
  • Mowiol (10%) or Gelvatol (15%)

    5. p-phenylenediamine may cause Cy-dye to fade, use n-propyl gallate instead.