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Star Republic: Guide for Biologists

Mounting medium

1. Add a drop (~20 ml) of the mounting medium to the slide.

2. Cover with cover slip.

3. (optional) Seal with nail polish.

4. Store at room temperature or -20 C.

Commercial

Vectashield

Vector

Vectamount

Vector

ProLong

Molecular Probes, antifade mounting medium

Aqueous medium

Polysciences

DPX

Polysciences

TBS mounting media

Fisher

Home made

Glycerol/PBS (9:1)

pH to 8.5-9.0 for fluorescence

Glyerine Jelly

4% gelatine, 25% glycerol, 0.1% phenol. Dissolve by warming and stirring. Store at 20 ˚C. Warm to 40 ˚C before use.

Apathy's

50% gum acacia (arabic), 50% sucrose, 10% CaCl2, 0.1% thymol. Dissolve by warming and stirring. Store at room temperature.

Fructose

15 g fructose, 5 ml water, dissolve at 60 C for 1-2 days. Store at room temperature.

Canada Balsam

Dissolve Canada balsam resin in xylene or benzene.

DPX

Distrene-80* (polystyrene) 10 g, Dibutylphthalate 5 ml, xylene 35 ml. Dissolve for several days. Store in dark bottle.

*Distrene-80 is a trademark name of a polystyrene. DPX from different vendors may differ in the polystyrene used.

See also Mounting medium --- anti-fade.