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Star Republic: Guide for Biologists

Counterstain
  • 1. Deparaffinize: xylenes 5 minutes.
  • 2. 100% ethanol wash
  • 3. 95% ethanol wash
  • 4. 70% ethanol wash
  • 5. Rinse thoroughly in water
  • 6. Dip twice in Hematoxylin (1-2 min.)
  • 7. Rinse thoroughly in water
  • 8. Dip twice in Bluing Reagent (30-60 sec)
  • 9. 70% ethanol wash
  • 10. 95% ethanol wash
  • 11. Eosin Y (1-2 minutes)
  • 12. 95% ethanol wash (x2)
  • 13. 100% ethanol wash
  • 14. xylene wash for 1 min
  • 15. Air dry.