Most stocks can be cultured by periodic transfer of adults to fresh
1. Tap the bottom of a bottle or a vial on the bench to shake flies away from
2. Remove the plug rapidly and invert the old culture over a fresh vessel.
3. Transfer some adult flies to the new vessel by gentle tapping.
4. Separate the two vessels quickly, shake flies to the bottom, and
5. Stocks kept at room temperature (25 °C) should be transferred every
20 to 30 days. Stocks kept at lower temperature can be transferred less