1. Examine new stocks for signs of mites. Using a microscope,
examine the medium surface and the vial walls.
2. Open a stock only if no mites are sighted. Transfer adult files to
a fresh vial. Keep the original and subculture in a quarantine tray.
3. Keep the original for about 20 days. Rechecking for mites every 5 to
4. If mites are found, keep infected cultures wrapped in benzyle benzoate-treated
cheese cloth. The cheese cloth is soaked in 10% benzyl benzoate in 95%
ethanol and air dried. Replace the cloths every 6 months.
5. Transfer adults to a fresh vial daily for about a week. Use the last
transfer to establish a new culture.
6. Infected cultures need to be wrapped in benzyl benzoate-treated
cheese cloth until they have been mite-free for three generations.