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Star Republic: Guide for Biologists

Distinguishing male from females

Edited by Chang Zhu
1. Anesthetize the files and exam the files under a dissecting microscope. Use a hairbrush (paintbrush) or feather for moving files around.

2. Looking for the following differences:

  • Males have sex combs on the front legs.
  • In older files the posterior part of the abdomen is quite dark in males than in females.
  • The tip of the abdomen is more rounded in males than in females.
  • In general males are smaller.
The most reliable difference is the sex combs.