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Star Republic: Guide for Biologists

Establish clean cultures from dechorionated eggs

1. Collect eggs using a hard agar plate.

2. Transfer eggs to a cheese cloth or filter paper socked in 50%-100% household bleach. Chorions will be dissolved within 3-5 minutes.

3. Wash eggs several times with water and transfer the eggs with a brush to a small piece of filter paper.

4. Wet the paper with water and place the filter paper with eggs on the surface of the medium in a fresh vial.

5. Place a strip of filter paper along the wall of the vial. Wet the paper with water to maintain high humidity in the vial until the eggs hatch.