1. CO2 gas: Frequently used in fly labs equiped with
2. Flynap: available from Carolina Biological Supply.
3. Ether: add a few drops of ether to a cotton ball, add the cotton to a bottle
containing flies, and cover the lid. Or use with the anesthetizer (or etherizer). Caution: ether is highly
flammable---don't allow open flames or electronic motors in your lab.
4. Cooling. Place vials containing files on ice for 5-10 minutes. After the
flies are not moving, dump onto a chilled surface such as petri dish on top