Synchronization of cells --- summary Method Cell type Stage of arrest Working Concentration Aphidicolin Mammalian cells early S 15 mM Hydroxyurea Mammalian cells early S 2.5 mM Mitotic shake-off Mammalian cells M N/A Nocodazole Mammalian cells M 50 ng/ml Serum starvation Mammalian cells G1 N/A Thymidine Mammalian cells S 2 mM a-factor Yeast G1 2 mg/ml Hydroxyurea Yeast S 0.2 M Nocodazole Yeast M 15 mg/ml