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Star Republic: Guide for Biologists

Synchronization of cells --- summary
Method Cell type Stage of arrest Working Concentration
Aphidicolin Mammalian cells early S 15 mM
Hydroxyurea Mammalian cells early S 2.5 mM
Mitotic shake-off Mammalian cells M N/A
Nocodazole Mammalian cells M 50 ng/ml
Serum starvation Mammalian cells G1 N/A
Thymidine Mammalian cells S 2 mM

a-factor Yeast G1 2 mg/ml
Hydroxyurea Yeast S 0.2 M
Nocodazole Yeast M 15 mg/ml