Adherent cells in the mitotic phase detach, can be collected by shaking.
1. Grow cells in a large flask to subconfluent (mid-log phase).
2. Shake-off mitotic cells by gentle shaking or tapping of the flask or
by gently washing with the warm medium.
3. Transfer the medium containing mitotic cells to a sterile tube,
store on ice.
4. Add fresh pre-warmed medium to the flask and return it to
5. Repeat step 2-4 every 10-30 minutes until enough cells have been