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Star Republic: Guide for Biologists

Synchronization of cells --- growth condition

Many type of cells can be synchronized by manipulating the growth conditon.

  • For cells that depend on serum for growth, starve the cells of serum for 24-48 hours, and then change to fresh media with serum.
  • For bacteria, fungi, algae grown to stationary phase, simply dilute/change to fresh medium.
  • Amino acid starvation.
  • Heat shock and temperature shift.