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Star Republic: Guide for Biologists

Detetion of DNA in PAGE --- UV shadowing

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1. Carefully remove PAGE after running and place on Saran wrap.

2. Place the gel on top of the fluor-coated TLC plate.

3. Visualize DNA bands by shining a hand-held shortwave UV source (254 nm) in a dark room.

4. DNA will be dark purple and the TLC plate will appear green.