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Star Republic: Guide for Biologists

Sequencing of GC-rich DNA

Virtualab Protocols (Chang Bioscience)
One or a combination of the following may help:

1. Add 5% DMSO in cycle sequencing reactions.

2. Add betaine to final concentration of 1 M in cycle sequencing reactions.

3. Manual editing of the sequence data based on the electropherograms.

4. Substitute dGTP with dITP in the reactions.

5. Running sequencing gels at higher temperature and/or in the presence of stronger denaturants.