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Star Republic: Guide for Biologists

Dephosphorylation of DNA (SAP)

Shrimp Alkaline Phosphatase (SAP) is active in virtually all restriction enzyme buffers and may be added directly to a restriction digestion.

1. After completion of the digestion, add 1 ml of SAP to the digestion mix.

2. Incubate at 37 C for 1 hour.

3. Inactivate SAP at 65 C for 15 minutes.