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Star Republic: Guide for Biologists

Replicate clones in 96 well plates

Virtualab Protocols (Chang Bioscience)
1. Clones (e.g. EST) are frequently stored in 96 well plates. If master plates are low density bacterial cultures, incubate master plates sealed over night at 37 C. This step is not necessary if the master plates are high density bacterial cultures.

2. To prepare replicate copies of the master plates, prepare sets of 96 well round bottom plates and add 100 ml of LB/10% glycerol containing antibiotics.

3. Spin the master plates briefly (1000 rpm for 2 minutes).

4. Inoculate each well with 5 ml of liquid culture.

6. Cover the inoculated 96-well plates with lids, seal with parafilm, and grow overnight at 37 C. These will be the working plates

7. Freeze working plates and store at -80 C.