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Star Republic: Guide for Biologists

DNA ligation (quick ligation)

The following protocol is for the Quick Ligation Kit from BioLab.

1.

Vector DNA ( 20-200 ng) 1 ml
Insert DNA ( 60-600 ng) ( 3 times vector) 1 ml
2X Quick Ligation Buffer 10 ml
Quick T4 Ligase 1 ml
ddH2O 7 ml

2. Mix and incubate the ligation at room temperature for 5 minutes.

3. Chill on ice. Don't heat-inactivate ligase. Transform or store at -20 C.