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Star Republic: Guide for Biologists

PCR cleanup (Qiaquick spin column)

1. (optional) Add 1 ml sodium acetate (3M pH5.2) to the PCR reaction.

2. Add 5 volumes of Buffer PB.

3. Place a Qiaquick spin column in a 2 ml collection tube.

4. Apply sample to QIAquick column.

5. Spin 30-60 seconds at 12,000 rpm.

6. Discard flow-through, add 750 ml PE Buffer.

7. Spin 30-60 seconds at 12,000 rpm. Discard flow-through.

8. Spin 1 minutes at 12,000 rpm. Discard flow-through.

9. Place column in a new tube. Add 50 ml EB buffer (10 mM Tris-HCl, pH8.5) or H2O to the center of the QIAquick membrane, let the column stand for 1 minute, and centrifuge for 1 minute at 12,000 rpm.