1. Sterile toothpicks or an inoculation loop are frequently the choice for
picking single clones. Toothpicks should be autoclaved and kept in a closed
bottle or beaker with aluminum foil cover.
2. Light a small flame and keep opened toothpicks, plates, or media near the
3. Select a single colony that is well separated from the others. If using toothpicks,
gently touch the colony with the tip of a sterile toothpick, and transfer
the cells to another plate by streaking on the plate or to a liquid culture by
dipping the tip with cells inside the media.
4. If using an inoculation loop, sterilize the loop by dipping in 70% ethanol
and then move the loop over the flame. Wait until the loop is cool.
Gently touch the colony with the loop, transfer cells to a different plate
by streaking or to a liquid culture by dipping in the media.