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Star Republic: Guide for Biologists

PCR cleanup (agarose gel)

Agarose gel purification of PCR products is necessary if there are multiple bands in the product and only one band is desired.

1. Run 10-20 ml of the PCR product on an agarose gel.

2. Visualize DNA under the UV light and identify the desired band.

3. Purify the PCR band using one of the following protocol: