1. 96 deep well plate is used for growing bacteria cells.
2. Bacteria is usually growing in 1.3-1.5 ml of rich medium
such as 2X YT broth + 70-100 ml/ml Ampicillin.
3. Longer time may be required. Incubation time ranges from 18-24 hours.
4. Higher shaking speed may be beneficial (250-350 rpm).