The low detection limit of Pico Green is ~ 1 ng/ml.
1. Make up a serial dilution of DNA standard (0-500 ng/ml).
2. Dilute the sample to concentrations between 50-400 ng/ml.
3. Mix 5 ml sample or standard,
1 ml Pico Green, and 94 ml TE
in cuvettes (bigger volume may be needed) or a plate. Mix.
4. Read with a fluorometer.